Cytometry and Cell Sorting
Flow cytometry is a powerful technology used to measure different aspects of a cell or particles in real time and high throughput. Measurements are acquired optically by using different lasers to detect fluorophores; multi-parameter analysis of up to 18 parameters can be measured at our facility. Applications include immunophenotyping, cell cycle, intracellular fluorophore detection (ex GFP, RFP), among many others. These assays can utilize cell sorting technology which physically isolates populations of interest for downstream applications including cell culture, RNA/microarray analysis, single cell cloning, and many more. Sorting of biohazardous material can be accommodated in our BSL2 or BSL3 facilites. Flow cytometry is adaptable and can be tailored to your particular inquiry; contact the core staff to discuss if this technology is the best choice for your research needs.
- Getting Started
- Instrument Reservations
- Instrument Reservations Help
- Instrumentation and Services
- Common Protocols
- Lab Policies
- Training, Events & Other Links
- Contact Us
- BELLEVUE RE-OPENING! Our Bellevue C&D facility is now operational! The FACSARIA can now be booked through our LIMS scheduler. All other Cytometry Core services are available.
- The Alexandria Center now has a FACSCalibur analyzer. It is located in room 843F. Please contact the core to have an account generated for you on the instrument computer.
- There is a Nexcelom Cellometer cell counter available in the Skirball core facility. This can be used to count, visually size cells and learn about the viability of your sample. Feel free to set aside an aliquot of your sorting material to take a look, we only need 10ul!
- IMPORTANT: FACSCalibur and FACScan Users. All FACSCalibur and FACScan users must check in with the lab BEFORE running samples on that instrument. The analyzer computers have been upgraded to OSX and Cellquest Pro. These upgrades will improve the speed and reliability of the software as well as offer more capabilities for data output and user organization/tracking. A new account must be set up on this machine before it will be available for use. Please contact the core to have one set up. Also, Cellquest Pro does have some significant differences from the older version so it is suggested that users meet with the core staff for a quick training.
- "Flow Cytometry 101" class is on June 3th from 9-12pm in the Smilow Multipurpose Room.
- LSRII Certification class is on April 18th from 10:00-12:00pm in Smilow room 701A.
Core email: Cytometry@med.nyu.edu
Core information phone: 212-263-5907
Staff hours 9:30am – 5:30pm weekdays
BSL-3 Sorting Facility