Immunohistochemistry (IHC) Core
The Immunohistochemistry Core, a component of the Experimental Pathology Shared Resource, has relocated to room 310 of Smilow on the main NYULMC campus as of Decemeber 2012.
The laboratory serves as an immunohistochemical antibody repository with a catalog of over 1,000 antibodies. Please click on the link to see the most recent version of the antibody catalog. Antibodies are in stock and available for use in the core laboratory for the collection of experimental immunohistochemistry data. The laboratory develops immunohistochemical protocols for new antibodies (commercial or proprietary) that have not been tested in fresh, frozen, fixed human or animal tissues and cells. In addition to performing manual labeling, the laboratory is equipped with two Ventana NEXES and one Discovery automated immunohistochemical staining instruments. The laboratory uses human and animal tissue micro-arrays for optimization and employs a wide variety of antigen retrieval methods for protocol development. The IHC core offers tyramide and polymer amplification methods (manual) and single or two color chromogenic immunohistochemistry. The laboratory has also commenced developing protocols and validating antibodies for immunoflorescence in frozen and FFPE tissue samples. Archival human tissues for creating TMAs are procured through the Biorepository Center. Animal tissue is collected an processed through the Histopathology Core Laboratory.
The IHC core laboratory faculty and staff are available to provide hands-on informal training, as well as guidance and support for the design and execution of experiments involving histopathology and immunohistochemistry of human and animal cells and tissues.
|Animal Antibody Sample Images||Human Antibody Sample Images|
Investigators interested in performing IHC related work are encouraged to contact the laboratory to request the most recent version of the core antibody catalog. This is especially important prior to purchasing antibodies as the staff has extensive experience with many of the antibody manufacturers, and may already have the antibody in its inventory.
Hurricane Sandy Update: We are happy to announce that most Experimental Pathology Core services are back up and running post-Hurricane Sandy. For the near future, we will be located in 2 spaces: 1) Skirball 5, Lab 6; and 2) Smilow 310. The locations of services and instruments are detailed below. If you have any general questions regarding the status of Experimental Pathology operations, please contact Cindy.Loomis@nyumc.org.
Smilow 3rd floor, Rm 310: Drop off and pick up of samples destined for paraffin processing, embedding, and/or sectioning as well as all samples for H&E and special histochemical staining, slide deparaffinization, coverslipping, and whole slide scanning. Rental instruments in Smilow include the Leica embedder, Ariol work station for image analysis, and Zeiss Axioscope for photomicroscopy. The Leica LMD6000 laser capture microscope will be available in mid-January. For consultations on whole slide scanning and laser capture microscopy, contact Lisa.Mara@nyumc.org.
Automated Immunohistochemistry services are available in Smilow 310, including access to the entire IHC antibody catalog. Due to space constraints we are only offering limited manual staining at this time. Slides and samples may be dropped off in Smilow 310. We are also pleased to announce that in collaboration with the Biorepository Center and Histopathology Core we are now creating paraffin tissue microarrays (TMA). To request a copy of the IHC antibody catalog or for more information on TMA’s, please contact Luis.Chiriboga@nyumc.org or call 347-712-0897. If you need assistance on initiating IHC projects or general guidance, please call 646-501-6934 or email Luis.Chiriboga@nyumc.org or Stephanie.Krauter@nyumc.org.
Skirball 5, Lab 6: Drop off and pick up of samples destined for cryo (frozen) processing and/or sectioning. Rental instruments in Skirball include a Microm cryostat, Leica microtome and Nikon fluorescence compound microscope. For consultations on histology projects, contact Mark.Alu@nyumc.org or Zahidunnabi.Dewan@nyumc.org (Zahid).
All requisitions for services and instrumentation reservations (Link to Instrument reservations page) must now be placed through our new online ordering system, LIMS. If you have any questions, call 212-263-3888 or 347-712-0806. Alternatively, email the Core at any of the emails above or Histopath.email@example.com.
New tissue sample drop off rules for paraffin embedding:
- Samples can be dropped off and picked up at Smilow 310 between 9-11 am and 1-3 pm.
- All samples must be brought in 70% ethanol or PBS in screw cap containers. Open containers, etc. will not be accepted.
- All containers must be taken back to your lab when you pick up your samples. If they are not picked up, they will be discarded or re-purposed by the Core.
SlidePath digital image database: the High Performance Computing facility is in the process of re-building and configuring our local SlidePath database system for managing whole slide digital scans. Until this is complete, we have several alternative options for viewing and analyzing both old and newly scanned images. Please contact Lisa.Mara@nyumc.org for instructions.
Seminars: Biotechniques and Leica present: “Digital Pathology: Expanding the Microscope Box”, free Webinar featuring Dr. Cindy Loomis, NYU Histopathology Core Director. To view: http://www.biotechniques.com/digitalpathology
NYU Langone Medical Center
550 First Avenue
New York, NY 10016
Phone: 646-501-6934, 347-712-0897