Microscopy Core Photo in Textbook
Dr. Fengxia (Alice) Liang and her colleagues in the Office of Collaborative Science (OCS) Microscopy Core will have a scanning electron micrograph of segmented filamentous bacteria (SFB) attached to intestinal epithelial cells from the terminal ileum of an 8-week-old Taconic B6 mouse published in the third edition of the college textbook, Microbiology by Slonczewski/Foster. Published by WW Norton, 99,000 copies of the textbook will be printed in September, and will also be available to students as an e-book. The artificially colored image has also appeared in a news article on the microbiome published last year in Nature Outlook by the science writer Lauren Gravitz and an earlier commentary in Nature by Ivalyo Ivanov and Dan Littman.
UltimateFocus Module for API PersonalDV Live Cell Imaging System
Microscopy Core upgraded the API PersonalDV live cell imaging system with UltimateFocus, an autofocus module that automatically maintains focus during time-lapse experiments. Axial fluctuation, which is caused by gradual changes to the focusing during live cell imaging, is always a major challenge to researchers. This phenomenon is termed as focus drift and it defines the inability of the microscopic system to maintain the chosen focal plane over a period of time. This results in loss of contrast, yielding “blurry” images with little information. UltimateFocus module utilizes a non-destructive IR laser to continuously maintain the sample position and focus. It can return sample’s z-position within 25nm and takes only 0.2 second for such correction. This upgrade remarkably improves the PersonalDV system as an essential tool for live cell imaging, thus allowing users to generate sharp, crisp time-lapse images
HyD Detectors for Leica TCS SP5 II Confocal Microscope
Leica TCS SP5 II confocal microscope has upgraded two of its four PMTs with new HyD detectors. HyD is a GaAsP hybrid detection system newly developed by Leica Microsystems. It significantly improves image quality and detection efficiency with deep contrast and super-sensitivity. Its high quantum efficiency, low dark noise, and large dynamic range make the Leica HyD a very versatile confocal detector. This upgrade, coupled with Tandem Scanner which is already installed, makes this system a fast, super-sensitive confocal microscope that allows high speed, high quality imaging. For more information of this upgrade, please contact Yan Deng in Microscopy Core.
Volocity Licenses Server
The imaging license server allows Volocity licenses to be accessed across the NYULMC network. You can view your cells in 3D and 4D with Volocity Visualization; find, measure, track and chart objects in your sample with a huge range of features in Volocity Quantitation, and use powerful algorithms in Volocity Restoration to eliminate noise and blur in your data. Please contact Yan Deng for further assistance.