The Philips CM200 cryoelectron microscope for high resolution structure determination with field emission gun is operated at 200 kV. The microscope is equipped with Gatan UltraScan 1000 2kx2k CCD camera (bottom-mounted) and a TV-rate image intensified camera (Gatan 626). We have several cryoholders (Oxford 3500 and Gatan 626). Digital images can be acquired either with the Gatan Digital Micrograph or through TVIPS EMMenu 3.0, the latter of which is used for collecting tomographic tilt series.
This microscope is distinguished by its Field Emission Gun (FEG) and Computer-controlled Sample Stage (CompuStage). The FEG drastically increases beam coherence and brightness, substantially improving image quality with much higher resolution. The CompuStage allows the automated image acquisition required for electron tomography and also allows users to memorize specific specimen locations when searching for the best place to record their data.
Main Features of the Microscope
|High Tension||20 - 200kV in 40-kV increments|
|Extractor Voltage||Set at 4 kV maximum|
|Magnification||50 - 750,000x|
|Camera Length||30 - 265mm|
|Column Vacuum||Ultimate Pressure = 2.7x10-5Pa (IGP = 18)|
|Focusing||0.3um-10mm(LM Range), 3nm-100nm(M/SA Range)|
|Spot Size||10um-200nm(LM Mode), 2.0um-40nm(Microprobe), 100nm-2nm(Nanoprobe)|