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EmailGE Typhoon Trio™
Features and Benefits
Courtesy of GE:
The GE Typhoon Trio™ has three lasers enabling excitation and visualization of a wide range of wavelengths. Applications include fluorescent stained protein gels (SYPRO Ruby, Deep Purple), fluorescent western blots (ECL Plus, ECL Plex), fluorescent nucleic acid stains (SYBR-green), 2D-DIGE (Cy2, Cy3, Cy5) etc. Gels and blots are positioned directly on a glass plate for scanning. The Typhoon is not recommended for straight chemiluminescence, but fluorescent alternatives such as ECL Plus, and Cy-dye labeled secondary antibodies do allow western blotting experiments to be analyzed. Images can be saved as TIFF files for manipulation in standard graphic software. Analysis is performed using ImageQuantTL software which is loaded on the Typhoon computer.
Useful Links
Standard Set of emission filters for the Typhoon Trio™ Imager
| Color | WaveLength | Light Source |
| Red Light | 632.8 nm | Helium - Neon Laser |
| Green Light | 532 nm | Solid State doubled frequency SYAG laser |
| Blue Light | 488 nm | Argon Ion Laser |
| Blue Light | 457 nm | Argon Ion Laser |
| Emission Filters | Description |
| 520 nm band pass | Cy2, ECL Plus, Fluorescein |
| 555 nm band pass | R6G, HEX |
| 580 nm band pass | Cy3, Tamra |
| 610 nm band pass | SyproTM Ruby, ethidium bromide |
| 670 nm band pass | Cy5 |
| 526 nm short pass | Fluorescein |
| 560 nm long pass | TRITC |
Sign Up, Questions, Training Requests
Interested in using the GE Typhoon Trio? Have additional questions or need training? Visit the Research Support Core (RSC) page to Sign Up or submit a Request Form for your queries and training needs.
