Rodent Genetic Engineering Core (RGEC): Forms
ES Cell Injection
All targeted clones must be MAP-tested prior to their introduction into any NYUMC animal laboratory. It is strongly recommended that all targeted clones be karyotyped prior to injection - contact the Director for further information.
- An aliquot of purified DNA construct in injection buffer (minimum volume 50µL); we will provide injection buffer.
- A photo of a gel clearly indicating the band to be injected.
- A Spec reading of submitted DNA.
- A copy of the Southern blot genotyping protocol which will be used to screen all putative founders.
Do not submit a rederivation requisition until imported mice have arrived into the NYUMC quarantine laboratory. Please obtain a Rederivation Importation accession number from DLAR and enter it onto the requisition form prior to submission.