Translational Research Core: Services
The Translational Research Core (TRC) offers a variety of fee-based services including specialized assays, molecular biology, specimen handling, SNP genotyping, data analysis, and maintenance of a centralized database for study samples.
The TRC has received the Pharsight Academic Center of Excellence Award since 2002, allowing access to the most current nonlinear modeling software.
1. Specialized Assays
The TRC develops and performs specialized quantitative assays using a variety of methodologies applicable to clinical specimens. Assays range from SNP genotyping, bronchoalveolar lavage, platelet activation assay, the measurement of serum/plasma levels of compounds of interest and/or secreted products of the immune system, to effects on peripheral blood mononuclear cells or tumors. The TRC performs Pharmacokinetic assays, preclinical, and in-vivo studies are conducted in accordance with cGMP. All assays undergo a rigorous validation protocol before being put to use.
- SNP genotyping (APOE, obesity, etc)
- Bronchoalveolar lavage
- Platelet activation assay
- CPCs, CECs - CTEP and MSKM
- PARP inhibition in PBMCs -NCI optimized ELISA assay -CTEP certification obtained for TRC
- CTEP lab-certified for TRC Core for CTCs and tumor tissue
- Gamma H2AX in PBMCs, CTCs and tumors
- LCMS PK
- HPLC assays in plasma and cells (dendritic, and tissue culture)
- ELISA assays for specialized cytokines and unique PD studies
- Development of new analytical assays for future clinical trials
2. Molecular Biology
- Synthesis of oligonucleotide primers for use in DNA sequencing
- Primer-directed mutagenesis
- cDNA or genomic DNA library construction
- DNA mobility shift assays
- User based DNA thermal cyclers for PCR and Taq polymerase sequencing
- ELISA plate reader for evaluation of cytokines
- DNA sequencing by dideoxy chain termination using either Sequenase, reverse transcriptase, Klenow and newer sequencing methods with Taq polymerase and thermal cycling
3. Training and Intradepartmental/Interdepartmental Research Coordination
- The TRC coordinates chemopreventive and pharmacological programs in the research lab and clinics and facilitates collaborations among NYU Clinical Cancer Center, CTSI, NYU Langone Medical Center, New York University, affiliates and non-affiliated institutions.
- The TRC teaches molecular biology techniques and principles to investigators, fellows, medical students and technical staff, including Southern, Northern, and Western analysis, labeling techniques, RNA and DNA isolation, polymerase chain reaction, ELISA, dideoxy DNA sequencing, recombinant cDNA or genomic DNA library construction and transfection of DNA into mammalian cells.
4. Data Analysis
- The TRC determines the pharmacodynamic and pharmacokinetic parameters of agents under study. Time-based sampling is obtained through the use of computerized modeling of the data. Data analysis involves pharmacokinetic modeling, drug-drug interactions, and quantification of immunohistochemical (IHC) data.
- The TRC is involved in initial real time processing of non-routine PK & PD samples and then hands it off to other cores when appropriate (Analytical Chemistry, Genomics, Clinical and Translational Science Institute, Tissue, Flow Cytometry and Cell Sorting Center).
5. Centralized Data Base for Study Samples
- The TRC maintains a centralized database to correlate results with treatment patients received during studies.
6. Cytokine analyses
- The TRC offers multiplexing of cytokine analyses, chemokines, growth factors and other proteins with two Luminex 200 platforms.