Translational Research Core: Instruments and Equipment
The TRC laboratories are located on the 6th floor of Bellevue C&D Hospital. Radiochemical work is conducted in the chemistry laboratory, adjacent to the PET/CT imaging facilities in the NYU Clinical Cancer Center.
A complex of 6 high-pressure liquid chromatography gradient apparatuses, run with the aid of 2 Axxiom 747 and 1 Pyramid PC-based computerized controller and data systems. These interactive systems allow complete automation of sample processing and analyses with the use of six WISP 717 (Waters) autosamplers. Two additional automated HPLC systems are available. A third, used for radiolabeled work specific to ongoing clinical studies in the chemistry laboratory, is located at the Cancer Institute. Chromatographic data are captured live and archived on disk drives allowing the ability to reprocess data as necessary. The data are backed up on network servers. The system allows for the programming of quality control methods with the use of daily external standards to calibrate the system and update the standard curve for calculation of areas of relevant peaks. Eight ultraviolet/visible, fluorescence (4) and electrochemical (1) detectors are available for peak detection. The measurement of radioisotope labeled drugs is achieved by the use of 2 BioScan flow detectors with specialized heads for beta and gamma emissions. Two photodiode array (Waters 990, Perkin Elmer 215) are available for sample characterization. Initial sample processing is carried out in either a laminar flow or a chemical hood or for radiolabeled material behind Capintec L-block shielding as required for optimal safety.
Molecular Biology Activities
All essential equipment required for molecular techniques and tissue culture including microfuges, speed-vac, ultra/high/low speed centrifuges, two BL-2 tissue culture hoods, PCR hood, shaker, circulating water baths, water baths, hybridization oven, thermal cyclers, computers, ELISA reader, biosafety cabinet, refrigerator, cold box, cytospin, 2 microscopes and three -80°C freezers. The refrigerator and freezer can only be used to store samples temporarily. Samples can be separated and aliquoted, provided written instructions are noted in the standardized orders.
Facilities for Sample Processing:
- Laminar flow hoods (2)
- Clinical, refrigerated low speed (2) and high speed (1) centrifuges
- Coulter cell counter
- Sample storage
- CO2 incubators (2)
- Egg incubator (1)
- -20C (4) and -70C/-80C (2) freezers
- Western blot-Stowal blot washer
- Slot blot
- ELISA (Microtek microtitor plate reader) and RIA immuno-assays
- PCR assays
- CAM studies
All laboratory data are correlated by patient ID numbers and date in a HIPAA compliant unique relational database that resides on a networked server. Other databases are employed for order tracking, inventory and freezer storage. Data are exported for statistical analyses using Statview or JMP operating on Macintosh computers. The pharmacokinetic data are calculated using a separate data calculating channel available on each PC/data controller to process the daily chromatographic analysis, and a Pentium IV "workstation" machine which also is able to run the chromatographic software (Pyramid) for data analysis of any of the PC collected chromatographic runs. The Pentium running under Windows-XP pro environment uses Microsoft Excel for standard curve analysis and unknown calculations. The pharmacokinetic data estimates are done using WINONLIN version 4.1.
Laboratory work station computers are current models of Apple (G5, G4 power PC, and G4 and Intel MacMini as well as Pentium IV PCs (2). Data are backed up overnight via RetrospectTM to a centralized server and backups are also stored offsite on DVDs.
The TRC employs the Codesoft custom label software with Brady unique sample collection tube labels that have good durability and legibility under a variety of storage conditions at both of our main laboratory sites.