The Vaccine and Cell Therapy Core offers the following Immune Monitoring services and equipment:

Services

• Processing and storage of PBMC/serum/plasma samples
• Immune Monitoring Assays:
  • Immunophenotyping
  • Peptide/MHC Tetramer Staining
  • Enzyme-linked ImmunoSpot Assay (ELISPOT)
  • Intracellular Cytokine Staining (ICS)
  • Multiplex Cytokine Analysis (Luminex, CBA)
  • Enzyme-linked Immunosorbent Assay (ELISA)
  • Cytotoxicity Assays (51Cr Release Assay, flow-based)
• Consultation on experimental and clinical trial design involving immune monitoring
• Production of MHC class I tetramers
• Develop and standardize new assays for immune monitoring

Equipment

• BD LSR II Flow Cytometer
• CTL ImmunoSpot reader
• Luminex 200 analyzer
• SpectraMax M2 multifunction plate reader
• Miltenyi AutoMACS cell separator
• GE Healthcare AKTA Basic chromatography system

BD LSR II Flow Cytometer

GE Healthcare AKTA Basic Chromatography System

Luminex 200